The Molecular Mechanism Of QI Ji Preparation In The Prevention Of Nonalcoholic Steatohepatitis Of Rats

Objectives: With the improvement of the standard of living, more and more people are suffering nonalcoholic steatohepatitis (NASH). The me chanism of NASH is complicated and still unclear. It appears that a 1 ot of factors are concerned with pathological factors of it. Thereint o, overabundance of free fatty acid (FFA), oxidative stress and inju res to cytokines (CKs) are the important pathological taches. Nuclear factor-κB( NF-κB) is eukaryocyte transcription factor relating with oxidative stress and CKs, which can modulate the expression of many inflammatory factors,which may have important effect on NASH. There a ren't any ideal medications to prevent NASH. Chinese traditional medi cine (TCM) possesses some superiority on it. My tutor, professor Zhan g Chi-zhi, realized in his clinical work that NASH may be caused by e ating fat and sweet excessively for a long time, which lead to phlegm and blood stasis generating inside, constrained liver qi, heat being transformed due to stagnation for a long time, yin essence being was ted secretly, coming into being that the pathological characteristic of which the nature is insufficient but the signs are looked like exc ess. The principle of its treatment should be to supplement liver and kidney, to clear away heat evil and disinhibit water. He chose QI Ji Preparation(made up of medlar fruit and silybin ) to treat it and ga ined good effects. Using the model of NASH recognized by the world an d linking to the evolution of modern research of NASH, we observed th e hepatic tissue expression of NF-κB/IκB protein and mRNA in the esta te of NASH and threw light on the mechanism of QI Ji Preparation to p revent it by the technique of pathology, biochemistry and molecular b iology in the research, to offer deep study of pathogenesis and preve ntion of NASH.Methods : the research was divided into two steps. The first step to study the effect of QI Ji Preparation on NASH (Expe riment I): Eighty clean graded male Spraque-Dawley rats, weight 190± 20g . ware feed 1W normally, then were randomly divided into 8 groups, namely normal control group (A), blank model group (B), QI Ji Preparat ion groups: low dose, common dose, high dose (C1,C2,C3), Compound Meth ionine and Choline Bitarte Tablets (CMCBT) group(D), medlar fruit grou...