The Effects Of Stromal Cell-derived Factor 1 And Platelet Factor 4 On The Ex Vivo Expansion Of UCB HSPCs And Homing-related Function Of Expanded HSPCs

Hematopoietic stem cell transplantation (HSCT) has been used to treat thousands of patients with leukemia, lymphoma, inborn immunodeficiency, metabolic disease, autoimmune illness, and other life-threatening hematogical diseases. Umbilical cord blood (UCB) stem cells are an exciting alternative to bone marrow (BM) and mobilized peripheral blood (MPB) stem cells for transplantation. This is due to UCB has higher proliferation and self-renewal potential, abundantly available and risk free collection, low chances of contamination with CMV and Epstein-Barr virus, transplantation associated with low incidence of acute and chronic graft vs host disease compared to adult bone marrow allografts, cord blood banks can provide HLA matched stem cells on demand. Although UCB has many theoretical advantages, the relatively low number of stem cells contained in a single unit is the major limitation in the use of cord blood for allogeneic HSCT. An optimized ex vivo expansion of UCB HSPCs would be an urgent resolution way for the widespread clinical application of UCB.The transplanted HSPCs possess the proliferation and self-renewal potential, and the ability to home to the BM microenvironment, which are two critical issues for the hematopoietic reconstitution and long-term engraftment after HSCT. There are many ex vivo expansion protocols have been extensively evaluated, in which the expanded HSPCs would sustain their proliferation and self-renewal potential. However, the homing-related characteristics and function were rarely evaluated in previous reports. Few studies were focused on expansion protocols, in which the expanded HSPCs would sustain their ability to home to the BM microenvironment.Our previous study demonstrated that short-term culture system, which consists of defined QBSF-60 serum-free medium with a simple early-acting cytokine...