| Objective To create the model of isolation, cultivation and multiplication of mesenchymal stem cells from New Zealand white rabbits and Wistar rats respectively, provide seed-cells for constructing tissue engineering blood vessel. Methods BMSCs were isolated and purified from the bone marrow of rabbits and rats by percoll (1.073g .ml-1) density gradient centrifugation and adhering to the culture plastic. After successful cultivation and amplification, the biologic characteristics were observed and their immunophenotypes were identified by flow-cytometry. Results Successfully established the model of isolation, cultivation and multiplication of BMSCs in vitro. BMSCs show fence appearance under phase-contrast micoscope. Rats' primary BMSCs were all positive for CD90, CD44 and all negative for CD31, CD45. Conclusion BMSCs cultivated in this experiment were sufficient to be used as seed-cells in constructing tissue engineering blood vessel for the further experiments.
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