Studies On The Second Metabolites And Bioactivities Of Two Fungi

In the investigation of active components from fungi, several hundred fungi were isolated from medicinal plants including Dendrobium Candidum, D. noble, Ganoderma lucidum and 44 fungal strains which accelerated the growing speed of host plant were selected for tests of antitumor activities. These 44 fungal strains were cultured and filtered into two fraction, mycelia and culture filtrate respectively. The methanol extracts of mycelia and 80% ethanol extracts of culture filtrates were offered to test the antitumor activities in vitro by MTT method. Consequently, a strain (No. BMS-9707) which was isolated from Ganoderma lucidum (Leyss. ex Fr.) Karst. and identified as Calcarisporium arbuscula preuss showed the antitumor activities that more than 90% tumor cells were inhibited when the concentration was 100μg/ml.Nineteen compounds including macrocyclic trichothecenes, cerebrosides and diterpene were isolated from the culture of C. Arbuscula (No. BMS-9707). On the basis of classical chemical methods and modern spectroscopic analysis (IR, UV, ORD, ¹H-NMR, ¹³C-NMR, DEPT, ¹H-¹H COSY, HMQC, HMBC, NOESY, EI-MS, FAB-MS, TOF-MS), the structures were determined. Among the compounds, there were four new compounds and ten compounds isolated from Calcarisporium genus for the first time.The new compounds belong to the class of macrocyclic thichothecenes,...