Establishment Of An In Vitro Model Of Parietal Cells And A Study On Their Secretory Mechanisms

Peptic ulcer is a common gastrointestinal disease. Gastric acid secreted by parietal cells plays an important role in the pathogenesis and progressions of ulcer, and has since been paid much more attention to. The regulation of parietal cell function is accomplished by a variety of factors including neuropeptides and neurochemical mediators and it is difficult to study the effect of any single factor on gastric acid secretion at tissue or organ levels. It is therefore necessary to establish an in vitro model of parietal cells. So far the method for isolating parietal cells has not yet been developed in this country, which retards the progression of parietal cell study, including the mechanisms of GI hormones as well as the pathophysiology and pharmacology of peptic ulcer.The current study was an attempt to upgrade the study in this field in our country.To establish the method of isolating parietal cells and characterizing its biological activity. Gastric mucosa was stripped bluntly and digested with crude collogenase and EDTA, The dispersed gastric mucosal cells were loaded and pumped into an elutriation chamber. After centrifugation, the cell fractions were collected, and were then subjected to Percoll density-gradient centrifugation for further purification. The viability of cells was defined with trypan blue staining, and parietal cells were identified with HE staining. Accumulation of ¹⁴C-aminopyrine in the parietal cells and acridine orange staining were used to assess the acid secretion function. The H⁺-K`+ATPase a-subunit gene expression was...