Effects Of E1A Gene On The Growth,Radio-Chemosensitivity Of Human Lymph Node Metastasis Cell Line Derived From Human Head And Neck Squamous Cell Carcinoma

Objective: To study the effects of ElA gene on the growth rate, tumorigenicity and radio-chemosensitivity of human lymph node metastasis cell line LN686 derived from the patient with human head and neck squamous cell carcinoma(HNSCC), and make a preliminary study for gene therapy of lymph node metastasis of HNSCC. Methods: The recombinant -pcDNA3. 1-E1A plasmid containing the 1. 77Kb ElA gene, was introduced into the metastasis cell line —LN686 by using liposome. The cells resistant to G418 were selected and LN686-vect (containing empty vector) and LN686-E1A (transfected with pcDNA3. 1-E1A) cells were obtained. The changes of growth rates, doubling times, and cell cycle were also studied. After LN686 LN686-vect and LN686-E1A cells were injected into nude mouse, the time of tumor formation, growth rates and weight of tumor were observed. LN686, LN686-vect and LN686-E1A cells were treated with cisplatin, paclitaxel , bleomycin , 5-FU for 24h and irradiation, respectively. Viable cells were quantitated by MTT assays. Modelling of gene therapy utilizing ElA gene was performed in nude mouse, and growth rates of tumor were observed. Distribution of cell cycle of...