Study On Improving Resistance Of Human Hematopoietic Progenitor Cells To Chemotherapeutic Agents In Vitro By Transduction Of Multidrug Resistance Gene

Objective:Chemotherapy is important for treatment of gynecological malignant tumors . The majority of patients with malignant trophoblastic tumor could be cured by chemotherapy alone . Other gynecological malignant tumors , such as ovarian carcinoma and cervical carcinoma , are also sensitive to chemotherapy .However , myelosuppression following repeated and high- dose chemotherapy , which results in reducing the dose of chemotherapeutic agents , shorting therapeutic course and increasing drug-resistance cases, is one of the major obstacles in the treatment of gynecological malignant tumor at present . It is important to explore effective ways of protecting marrow cells in chemotherapy for improving the outcome of chemotherapy in patient with malignant tumors. Multidrug resistance gene (mdrl) encodes for a 170 kD transmembrane glycoprotein , P-170 ,which pumps a number of cytotoxic chemotherapeutic agents , such as Taxol ,Doxorubicin , VCR and VP16 , from intracellular to the extracellular compartment of cell , thus to generate drug-resistance of cells. In this study , human mdrlgene was transferred into human CD34~+ cells . Furthermore , some influencing factors in gene transduction and chemoprotection for hematopoietic progenitor cells...