In order to discover new antitumor agents from plant resources, Saccopetalum prolificum (Chun et How) Tsiang, a species of genus Saccopetalum (Annonaceae) growing in Hainan Province, China, was subjected to phytochemical studies. 25 compounds, including lactones, alkaloids, flavonoids, sesquiterpenes, steroids have been isolated from the roots of the plant, among which 7 were new compounds, named as Saccopetrin A (1), Saccopetrin B (2), Saccopetrin C (3), Saccopetrin D (4), Saccopetrin E (5), Prolifine (6) and (6'-O-margaroyl)-sitosterol-3-O-β-D-glucoside (7), respectively. The known compounds were 6-Hydroxyonychine (8), Isooncodine (9), Discretamine (10), Liriodenine (11), Lanuginosine (12), Clovanediol (13), Quercetin (14), Kaemferol (15), Quercetin-3, 4', 5, 7-tetramethylether (16), Quercetin-3, 3', 7-trimethylether (17), Quercetin-3, 4', 7-trimethylether (18), Quercetin-3', 5-dimethylether (19), Quercetin-3', 4'-dimethylether (20), Quercetin-3', 7-dimethylether (21), Quercetin-3, 3', 4', 5, 7-pentamethylether (22), Kaempferol-3, 7-dimethylether (23), Protocatechuic acid (24) and P-Sitosterol (25). Their structures were elucidated by means of various modern spectroscopic techniques, X-ray crystallographic analysis and chemical methods.Saccopetrin A~D (1~5) were a class of novel y-lactones having triple and/or double bonds in the aliphatic chain isolated from Annonaceae plant for the first time. The absolute configuration of saccopetrin A (1) was established by chemical transformation and Mosher method. This is the first determination of the absolute stereochemistry of γ-lactone applying Mosher method.The antitumor test in vitro against human tumor cell lines indicated that...
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