The Implication Of The Biological Indices Examination Of Bone Marrow Cells In Myelodysplastic Syndromes

Background and objective:Myelodysplastic syndromes(MDS) is a heterogeneous group of hematopoietic stem cell disorders characterized by marrow cell dysplasia and ineffective hematopoiesis, with a high risk of transformation into acute myeloid leukemia(AML). The current FAB classification scheme for MDS, based on marrow cell morphology and blasts percentages, subdivided patients into five subgroups However, within the individual subgroups, especially in RA and RAS, there is still great heterogeneity in terms of disease course, patients survival and leukemia transformation. To study the biological nature of the hematopoietic cells in MDS is of great significance for clarifying the essence of MDS as well as for the diagnosis, prognosis and treatment of MDS patients. We investigated the chromosomal karyotypes, in vitro CFU-GM growth patterns, proliferating cell nuclear antigen(PCNA) expression, cell cycle kinetics and hematopoiesis clonality of bone marrow cells from 37 MDS patients, two post MDS AML(MDS/AML) patients and 11 normal controls. Methods:R banding technique for karyotype analysis, in vitro semi-solid culture for CFU-GM growth patterns, flow cytometry after monoclonal antibody labeling for PCNA expression, flow cytometry after in vitro bromodeoxyuridine(BrdU) incorporation for cell cycle kinetics analysis, and X-linked HUMARA gene polymorphism assay for hemopoiesis clonality analysis. Results: 1. Twenty nine MDS patients were successfully cytogenetically...