Experimental And Clinical Studies Of Pulmonary Artery Perfusion With Hypothermic Solution On Lung Functions During Cardiopulmonary Bypass

一. Effects and comparison studies of pulmonary artery perfusion using three kinds of solution on morphology and functions of canine lung.Background: The inflammatory response and higher temperature of lung tissue during cardiopulmonary bypass can result in lung injury. This study was to evaluate the protective effect of pulmonary perfusion with hypothermic antiinflammatory solution on lung functions after cardiopulmonary bypass.Methods: Eighteen adult mongrel dogs were randomly divided into three groups. The procedure was carried out through a midline sternotomy, cardiopulmonary bypass was established using cannulas placed in the ascending aorta, superior vena cava and right atrium near the entrance of the inferior vena cava. After the ascending aorta clamped and cardioplegic solution infused, right lung was perfused via a cannula placed in the right pulmonary artery with 4℃ lactated Ringer's solution in lactated Ringer's group (n=6) , with 4℃ PGE1 solution in PGE1 group(n=6) and 4℃ aprotinin solution in aprotinin group(n=6). PGE1 solution consisted of PGE1, anisodamine, L-arginine, glucose-insulin-potassium and phosphate buffer. Aprotinin solution consisted of aprotinin and other components were the same as PGE1 group except prostaglandin El. Plasma malondialdehyde(MDA), white blood cell counts and lung function were measured at different time point before and after cardiopulmonary bypass, lung biopsies were...