The Study On Preservative Effects Of Cardioplegic Solution Supplemented With Diazoxide And Fructose-1, 6-Diphosphate On Isolated Rat Heart

Background: Heart transplantation is the most effective treatment for end-stage heart disease and has become a standard therapy in recent years. Successful heart transplantation depends on the high-quality preservation of donor hearts. Although many advances have been made in heart preservation, current hypothermic heart preservation still has many defects, one of the practical problems encountered in heart transplantation is the limited preservation time of the donor heart, which is usually 4-6 hours. How to maintain energy supply and to alleviate ischemic reperfusion injury is of great importance for heart preservation.Recent studies suggest that: opening mitochondrial ATP-sensitive potassium channel (mitoKATP) has cardioprotective effects on ischemia-reperfusion injury. As an important intermediate product of glycolytic pathway, Fructose-1,6-diphosphate(FDP) can increase energy supply of ischemic cells, alleviate ischemia injury, and improve function recovery of the heart. Microperfusion has been shown to be a promising technique for heart preservation. Therefore, we suppose that adding DZ and FDP to St.Thomas II solution, would improve the effects of heart preservation, and prolong the storage time limit combining with microperfusion technique.Objective: To investigate the protective effects of St. Thomas II solution supplemented with DZ and FDP on myocardial energy metabolism, myocardial apoptosis and endothelial cell injury of hypothermic preservative rat heart; To evaluate the effects of the new solution combining with microperfusion technique on prolonging the storage time.Methods: Isolated rat heart Langendorff model and working model were established. The study consisted of three parts: In the first part, 48 SD rats were divided into six groups randomly: STH group (St.Thomas II solution); D group (St.Thomas II solution +...