| Lung cancer is the leading cause of cancer death in the world. The cure rate remains <15% despite some advances in chemotherapeutic agents [2]. HER-2/neu gene also called erbB2,is a member of the epidermal growth factor receptor (EGFR)family and encodes a 185 kDa protein with tyrosine kinas activity and shows partial homology to epidermal growth factor receptor.The HER2/neu protein can dimerize with other members of the EGFR family and serves as strong oncogenic signals.The HER2/neu oncogene is over expressed in 30% of Non small cell lung cancer (NSCLC), and its signaling pathways play critical roles in tumor occurring, cancer cell growth, differentiation, metastasis, anti-apoptosis and resistance to chemotherapeutic agents.RNA interference (RNAi) is the phenomenon of sequence-specific, post- transcriptional gene silencing (PTGS) triggered by double-stranded RNA (dsRNA). RNAi exists in extensive organisms such as Caenorhabditis elegans, plants, mammalian, and is regarded as the genome's immune system. RNAi is now recognized as a mechanism for cellular protection and cleansing: it defends the genome against molecular parasites such as viruses and transposes, while removing abundant but aberrant nonfunctional messenger RNAs, as well as regulate developmental programs in a sequence specific manner. Now RNAi has developed into a novel gene-blocking tool and used to knockdown specific gene expression efficiently in various species. Its application includes screening new genes, functional genomics and virus and cancer gene therapy in which great achievement has been made. However, there is little application report about RNAi targeting HER2/neu gene in NSCLC. AimTo construct RNA interference vector to down-regulate HER2/neu gene and study the siRNA against HER2/neu effect on the cell cycle, tumor growth, and colony forming capability of lung adenocarcinoma cell line SPC-A-1 in vitro and tumor growth in athymic...
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