The Change Of Cochlear Cells Apoptosis And Its Related Genes' Expression In Guinea Pig Following Lower Intensity Blast Deafness

With the increase of application of industrial explosive substances and explosive weapons,the incidence of blast deafness is getting higher. It's caused by composite etiological factors,but the mechanism of deaf hasn't been completely explained. Now there isn't any effetive method to cure the injury of inner ear which caused by blast deafness. It has been shown from the recent studies that cochlear apoptosis is the the main modes of cell death which is cause by noise and aminoglycoside antibiotic. Caspase-3 plays an important role in cochlear development and deafness. Apoptosis-related protein Bcl-2 and Bax expresses and produces a marked effect in senium Meriones cochlear. But whether the cochlear apoptosis and related genes participate in the lower intensity blast deafness is not clear yet. This study is to clarify the regulating mechanism of apoptosis of guinea pig cochlear cells following lower intensity blast deafness (including the cochlear apoptosis and Caspase-3 related gene process express,activation,regulation,etc.) in order to provide theoretical evidence of clinic pevention and cure for neurosensory deafness caused by blast deafness. The experiment was divided into three parts.The first part was to establish an experimental model called lower intensity blast deafness of guinea pig: using the YBD-2000 blast wave generator,impacting the middle ear straightly with lower intensity compressed air to simulate lower intensity blast deafness, then dissectting the guinea pig's middle ear,testing the threshold of hearing,and observing the cochlea under light microscope (LM)and electron microscope(EM). The second part:approaching the changes of cochlear apoptosis and related genes following lower intensity blast deafness of guinea pig: based on the model of lower intensity blast deafness, using TUNEL,fluorescent staining and EM to detect whether apoptosis is a style of the cochlea injury of lower intensity blast deafness. Then using immunohistochemistry, In-situ hybridization histochemistry and Western Blot technique to detect the changes of Caspase-3, Bcl-2,...