| BackgroundBurns and trauma can not only lead to a series of pathological changes in the body, but also cause different degrees of systemic responses, including cellular adherence, signal transduction, proliferation and differentiation.Wound repair after burns and trauma is a complicated process, which have close relationship with the regulation of cell proliferation and cellular signal transduction. The cell proliferation and differentiation depends on the regulation of cell cycle. So, the research on the cell proliferation and differentiation is vital for the prevention of scar formation and wound healing.hCASK (human calcium/calmodulin-dependent serine protein kinase)is a member of the membrane associated guanylate kinase (MAGUK) family, which is consist of a group of conserved cytoskeletal proteins. CASK, as a scaffolding protein, participates in intracellular signaling pathways. CASK may play an important role in cytoskeleton assembly, signal transduction, cell junction formation, cell proliferation, et al.Our previous studies have shown that ECV304 cell proliferation was restrained, and the expression of p21WAF1/CIP1 and p16INK4a were up-regulated by the induction of hCASK.However, the mechanism is not clear.Recently, we have confirmed that hCASK bound to Id1 through its GUK domain, and Id1 might be involved in the cell growth mediated by CASK. CASK seemed to be an upstream signal of Id-bHLH pathway that regulated cell cycle and proliferation of ECV304 cells.Id1 family of helix-loop-helix(HLH) proteins does not possess a basic DNA binding domain and functions as a dominant-negative regulator of basic DNA proteins through the formation of inactive heterodimers with intact bHLH transcription factors. Id family has been implicated in regulating a variety of cellular processes, including cellular growth, differentiation, apoptosis, senescence, neoplastic transformation, but it is not well elucidated on the upstream signal of Id protein. |
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