Construction And Application Of Cell Lines Expressing HCV NS3/4A Serine Protease And Recombinant Caspase-3

HCV (Hepatitis C virus) is the causative agent of Hepatitis C which leads to the chronic hepatitis. Over 170 million people globally were infected by HCV, in China there were 40 million people. So the HCV infection has been a major worldwide health problem. To date, there is no vaccine and sepecific therapy available.With the successful use of small-molecule inhibitors of the human immunodeficiency virus (HIV) viral protease and reverse transcriptase in the clinic, the research anti-HCV targeting the protease and RNA polymerase were the focus in these years. However, lack of small animal model and efficient cell culture of HCV, the research and development of effective antiviral therapeutics against HCV has been impeded . Recently , the assay based the virus-encoded enzyme is often as the subrogate. It is conceivable in...