Screening Of Immunopotentiators And Study Of Related Mechanism

We got two immunopotentiators , ginsenoside-F3 and MTC-O1, from 26 ginsenosides and 56 MDP-Taxol conjugates by mouse spleen cell proliferation model and mouse peritoneal macrophages TNF-a model. Panax ginseng has been a valuable and important tonic medicine in many Asian countries such as Korea, China, and Japan. The main components of P ginseng are known to be the ginsenosides that are divided into two types, protopanaxadiol ginsenosides (PPDGs) and protopanaxatriol ginsenosides (PPTGs), by structural classification. To date, it has been reported that many kinds of ginsenosides play an important anti-inflammatory and immunomodulatory role by affecting cytokine production, lymphocyte proliferation. Ginsenoside-F3, an important component of PPTGs (an minor saponin in the leaves of Panax ginseng), was screened for its immunoenhancing activity from 26 ginsenosides. Further investigation of action mechanisms showed that ginsenoside-F3 at 0.1-100 μmol/Lwas found to not only promote the murine spleen cell proliferation, but also increase the production of IL-2 and IFN-γ, while decreased the production of IL-4 and IL-10 from murine spleen cells with the maximal effect at 10 μmol/L. RT-PCR analysis displayed that ginsenoside-F3 enhanced the IFN-γ and T-bet mRNA expression and decreased IL-4 and GATA-3 mRNA expression. EMSA experiment showed that ginsenoside-F3 10 μmol/L enhanced the NF-kB DNA binding activity induced by ConA in murine spleen cells, so ginsenoside-F3 has immunoenhancing activity by regulating production and gene expression of type 1 cytokines and type 2 cytokines in murine spleen cells.Paclitaxel (Taxol), originally isolated from the bark of the Pacific Yew tree, Taxus brevifolia, has potent antiproliferative activities against neoplastic...