In Vitro Evaluation Of Photooxidized Bovine Jugular Vein

Objectives: To evaluate the biological characteristic of valved bovine jugular veins stabilized by dye-mediated photooxidation.Method: The fresh valved bovine jugular veins were obtained from slaughterhouse and stabilized by dye-mediated photooxidation. The glutaraldehyde-treated veins and fresh veins served as controls. The samples were subjected to protein extraction assay and pepsin digestion assay, and analyzed by polyacrylamide gel electrophoresis. The photooxidized tissues were sterilized by iodine based sterilant, the effectiveness of sterilization was tested. The sheep jugular vein endothelial cells were cultured on the surface of tissue sample to assess the cytotoxicity of the sample. The human blood were incubated with th e sample extractant to see if the tissue sample cause hemolysis. The extracant of tissue were injected into the mice, and the signs of acute systemic toxicity including lethargy, hyperactivity, mortality, or body weight loss were recorded. Test and control extractans were injected into opposite sides of the shaved backs of New Zealand White rabbits, observations for erythema and edema were made. The rat subcutaneous model for assessment of tissue calcification and immunologic properties was used. Calcium levels were determined by elemental analysis on dried...