Screening Susceptible Population From Post-SARS Osteonecrosis

Objective To study the coagulation and fibrinolysis change of post-SARS osteonecrosis patients, investigate the etiology of post-SARS osteonecrosis, and select the sensitive molecular symbols for early diagnosis and screening the high-risk susceptible population.Methods The studied subjects were divided into two groups. 61 post-SARS osteonecrosis patients were one group, and 52 healthy people were as normal controlled group. Empty stomach blood samples from cubital veins were collected from both groups. Activated partial thromboplastin time(APTT), protein C(PC), antithrombin III(AT-III), plasminogen activator inhibitor(PAI), activated protein C resistance (APC-R), plasminogen(PLG), von Willebrand factor(vWF), D-Dimer (D-D), fibrinogen(Fib), lipoprotein(a) (Lp (a) ), apoprotein-B(Apo-B), homocysteine(HCY), IgA, IgG and IgM of anticardiolipin antibodies (ACL) types were examined by means of enzyme linked immunosorbent assay (ELISA), immunoturbidimetry assay, et al. Factor V Leiden and prothrombin G20210A mutation were detected by PCR, and PAI-1 4G/5G polymorphism was detected by PCR + Solid phase oligonucleotide assay (SPOLA).