Studies On P16 Gene DNA Promotor Methylation And The Expression Of Chemokine Receptors In Patients With SLE

Objectives:To investigate the state of p16 gene DNA methylation in plasma from the patients with Systemic Lupus Erythematosus (SLE) and its clinical. Materials and Methods:The methylation state of p16 gene promoter was measured by methylation specific PCR (MSP). A region containing CpG sites in p16 gene promoter was amplified by strand-specific polymerase chain reaction (PCR) after sodium bisulfite treatment.72 SLE cases and 20 healthy controls were enrolled. The state of DNA methylation in plasma and blood cells was detected and the corresponding clinical records were analyzed.Results:1, p16 gene hypermethylation in SLE patients was showen by plasma DNA detection.(1)There was a significant difference between SLE patients(29/45, 64.44%) and healthy individuals(1/20, 5%) (x~2=19.687~b; p=0, p<0.001; OR=34.44, 95%CI [19.25 ~ 49.63]) .It was found that the rate of DNA hypermethylation in active SLE patients was higher (20/24,83.33%) than that in inactive SLE patients (9/21,42.85%) (x~2= 8.008~b; P=0.005, <0.01; OR=6.67; 95%CI [2.05 ~ 11.29]). Only one healthy individual was detected DNA hyermethylation in plasma.