Effect Of Autologous Transplantation Of Bone Marrow Mononuclear Cells And Peripheral Endothelial Progenitor Cells By Percutaneous Intracoronary Artery Injection Myocardial Ischemia-Reperfusion Injury In Mini-swine Model

As a consequence of myocardial infarction, irreversible damage occurs to myocardial tissue. The adult myocardium cells do not have the capacity for self-renewal. Therefore, areas of myocardial scarring may compromise myocardial performance and may lead to expansion, thinning and remodeling of left ventricle, which is leading cause of ischemic heart failure and cardiac death. In recent years, efforts to treat severely compromised heart have been made. Cell regeneration may be a new and reasonable therapeutic method. The experimental research suggest that cardiac transfer of stem cells and progenitor cells can have a favorable impact on tissue perfusion and contractile performance. But the underlying mechanism of stem cell therapy needed to be elucidated.This research fell into four parts. Based on the creating of a standard mini-swine model of myocardial ischemia-reperfusion injury and the culture of endothelial progenitor cells (EPC), the authors compared the efficiency of two kinds of cells and the growth and differentiation of autologous bone marrow mononuclear cells (BM-MNC) and peripheral EPC transplanted into myocardial ischemia reperfusion injury region by percutaneous intracoronary artery injection.Part 1Silk Suture Ligating to Create a Standard Mini-swine Model of MyocardialIschemia-Reperfusion InjuryThe purpose of this study was to create a standard mini-swine model of myocardial ischemia-reperfusion injury by silk suture ligating for the research of stem cell.26 mini-swine were randomized into model group (n=25) and sham group...