| 1. The role of MCP-1 and MIP-2 in the pathogenesis of acute necrotizing pancreatits in ratsObjective To explore the potential role of MCP-1 and MIP-2 on the pathogenesis in the early acute pancreatitis(AP), and to study the effect of NAC on MCP-1 and MIP-2. Methods Thirty-five SD rats were randomly divided into three groups: sham-operation group,acute necrotizing pancreatits(ANP)3h,6h,12h group and NAC-pretreated 3h,6h,12h group. ANP were induced by retrograde injection 4% sodium taurocholate into the biliopancreatic duct. NAC-pretreated group were given 500mg/kg intraperitoneal injection. The levels fo plasm amylase, malondialdehyde (MDA) and myeloperoxidase(MPO) in the pancreas were tested. Pathological changes of the pancreas were observed. The expression of MCP-1 mRNA and MIP-2 mRNA were analyzedd by RT-PCR. Immunohistochemistry of NF-κB and MIP-2 were examined . Results Compared with SO group, the levels of plasm amylase, MDA and MPO in the pancreas significantly increased in ANP group(P<0.05 or 0.01). The expression of MCP-1 mRNA and MIP-2 mRNA were upregulated obviously in ANP group. There were strongly expression of protein of NF-κB in neutrophils, and MIP-2 in macrophages in ANP group. The expression of MCP-1 mRNA and MIP-2 mRNA were correlated with the severity of pancreatic injury. Compared with ANP group, the levels of plasm amylase, intrapancreatic MDA and MPO, and the expression of MCP-1 mRNA and MIP-2 mRNA in the pancreas markedly decreased in NAC-pretreated group. The expression of proteins of NF-κB and MIP-2 in NAC-pretreated group were significantly lowered than those in ANP group. The pancreatic injury obviously ameliorated in NAC-pretreated group as compared with ANP group. Conclusions The chemokines of MCP-1 and MIP-2 are expressed at the early stage of acute pancreatitis. Both of them might play an important role in the pathogenesis of AP. NAC might have benificial effects on AP through downregulation...
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