Constructions Of New Optimal RAAV Vectors And Applications In The Gene Therapy Of Lung Cancer

PART IRole of Cellular FKBP52 Protein in Hydroxyurea Treatment-Mediated Increase in Transduction Efficiency of Recombinant Adeno-associated Virus 2 VectorsWe have reported that tyrosine-phosphorylated forms of a cellular protein, FKBP52, inhibit the second-stranded DNA synthesis, leading to inefficient transgene expression from recombinant adeno-associated virus 2 (AAV) vectors. To further explore the role of FKBP52 in AAV-mediated transduction, we established murine embryo fibroblasts (MEFs) cultures from FKBP52 wild-type (WT), heterozygous (HE), and knockout (KO) mice. Conventional AAV vectors...