The Rapid Diagnosis Of Central Nervous System Bacterial Infection By Oligonucleotide Microarray Technology

IntroductionCentral nervous system bacterial infection is a commom disease . In recent years, because of the improvement of clinical diagnosis , vaccine inoculation and the modern antimicrobial therapy. , the fatality of this disease has been decreased. However, the prevalence of bacterial meningitis is obviously increased because of several factors such as the increased flowability of population , severe environmental pollution and the higher increased traffic accidence . Now bacterial meningitis is still a "top 10" infectious cause of death worldwide, and about 25%~50% of the survivors have neurologic and other sequelae of the disease such as seizure, hemiparesis, aphasia, dementia and so on. The most common pathogenic bacteria are Neisseria meningitides, Streptococcus pneumoniae and Hemophilies influenzae . Cerebral tuberculosis is seem to be rekindled in recent decade. To reduce death or permanent neurological sequelae as much as possible, a fast and correct diagnosis is of the utmost importance. The current "gold standard "for the diagnosis of bacterial meningitis is bacteria culture of cerebrospinal fluid (CSF). However, this approach might have some disadvantages. Some bacteria are very difficult to grow in nutrient medium and the results of culture may only be available after 24 to 48 h . Moreover, this methods sometimes have low sensitivity, especially when antibiotics have been given to the patient prior to lumbar puncture. The positive rate of bacterial culture is only 3.1%. Therefore, at present , the diagnosis of bacterial meningitis is usually based on the clinical presentation of the patient and the findings in CSF including cytology, biochemistry and immunologic test. To some extent, abusing antibiotic has become...