The Effect Of Leukemia-Related-Antigen-Pulsed Dendritic Cells Associated With Double Suicide Gene System In Allogeneic Bone Marrow Transplantation

Part I Experimental study on immunotherapy with leukemia-related-antigen-pulsed dendritic cells in leukemic mice modelObjectiveTo explore the feasibility and efficiency of immunotherapy with leukemia-related-antigen-pulsed dendritic cells(DCs) in leukemic mice model after allogeneic bone morrow transplantation (Allo-BMT) and investigate its' reasons.MethodsMature DCs were proliferated from bone marrow mononuclearcells (MNC) by adding mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) and interleukin-4 (mIL-4).3 days later, they were pulsed with frozen thawing L7212 leukemia-related antigen. In 6^th day .observed the morphous of mature DC by electron microscope and phenotype by FCM. 2 ×10⁷ bone marrow cells from a male BALB/c mice were transplanted into the myeloablative irradiated female 615 mice which received a single subcutaneous injection of 1×10⁶ L7212 leukemia cells 3 days before。 They were divided into control group (A),T cells group (B) , DC+T cells group (C) to receive immunotherapy in 14^th day after Allo-BMT .We observed...