Intramyocardial Electrocardiogram In Diagnosis Of Acute Rejection After Heart Transplantation, A Experimental And Clinical Study

1. Rat IMEG model study.Objective: Canine heterotopic heart transplantation intramyocardial electrocardiogram (IMEG) model has been developed for the study of IMEG monitoring. But the expensive price of canines has limited the widespread use of this model. More importantly, sampling error of endomyocardial biopsy affects experimental result. Availability of inbred strains, natural resistance to infection, and economy make the rat an ideal IMEG animal model. Technical problem of inserting intramyocardial electrode in rodent hasn't been resolved. This study is to seek a simplified technique of rat IMEG model.Methods: The rat heterotopic neck heart transplantation technique used has been thoroughly described previously. A 15 centimeters long enamelled copper wire, which diameter is 0.09 millimeter, is used as intramyocardial electrode. Each end of enamelled copper wire was burnt to eliminate 4 millimeters long enamel out of copper wire. Prepared enamelled copper wire was led through a 26G infusion needle and was everted over cusp about 1 millimeter. After having finished preparation of recipient and donor heart, the infusion needle with...