Determination Of Differentially Expressed Proteins And It's Significance Among Chronic Sinusitis,Nasal Polyps And Normal Nasal Mucosa Tissue

Chronic sinusitis and nasal polyps are both chronic nasal mucosa inflammation. In recent years more and more basic research has confirmed that etiological factors of chronic sinusitis and nasal polyps are multiple , and multiple cells,genes, proteins and signal transduction pathways are involved . Protein is the direct performer of vital movement. With the development of new high-flux experiment technology—proteomics, we could wholy, dynamically and interrelatedly investigate the differentially expressed proteins among chronic sinusitis, nasal polyps and normal nasal mucosa tissue. In 2004 we had initially performed PH3-10 gradient 2-DE of chronic sinusitis, nasal polyps and normal nasal mucosa tissue, and found isoelectric point ( PI) of most proteins is between PH 4-8. So in this experiment, we performed PH4-7 gradient 2-DE, in order to increase resolution and discover low abundance proteins. Then some differentially expressed proteins were validated by immunohistochemistry. At last significant proteins were linked with clinical curative effect, and we utilized rank correlation and logistic regression analysis (SPSS 10.0) to statistics objective indicators possibly employed in the clinical classification and prognosis judgement of chronic sinusitis and nasal polyps.Chapter 1 Determination of Differentially Expressed Proteins and It's Significance among Chronic sinusitis, Nasal Polyps and Normal Nasal Mucosa TissueObjective: To investigate differentially expressed proteins among chronic sinusitis, nasal polyps and normal nasal mucosa tissue by means of proteomic technology (PH4-7 gradient 2-DE).Methods: Proteins extracted from chronic sinusitis, nasal polyps and normal nasal mucosatissue were separated and the differentially expressed proteins were identified by series of proteomic tools, including immobilized PH4-7 gradient two-dimensional sodium dodecyl sulfate...