The Research Of The Effect Of Simvastatin On Pregnancy-associated Plasma Protein-A Expression And Its Clinical Significance In Vascular Injury And VSMC

[Objectives] To investigate the change of PAPP-A and CD40Lin rat carotid artery after injured by brief drying with a gentle stream of air along the lumen of the vessel, and study the relationship between PAPP-A and CD40Lwith the intimal hyperplasia; And explore the mechanism of the restenosis and regulation of PAPP-A expression.[Methods] 20 male SD rats were divided into two groups, the placebo group and simvastatin group. All rats' carotid artery were injured by brief drying with a gentle stream of air. The left carotid artery and control group(5 rats) were used as control.Rats were killed at 1 week and 2 week after injury, the blood and carotid artery sample were got to be studied. The intimal hyperplasia was studied by histological study. The expression of PAPP-A was measured by immunohistochemical method. The CD40L in artery tissue was determined by ELISA method. [Results]1. Artery injury at 1 week, the intimal area increased, but lumen's area decreased, the area of medial layer didn't change. No change was observed in control arterys.2. after artery injury, PAPP-A expression increased at 1 week later and still increased at 2 week later. CD40L was the highest at 1 week, then decreased, but could not be observed in control arterys.3. Compared to placebo group, simvastatin can inhibit the vascular intima proliferation The expression of PAPP-A and CD40L were also decreased significantly.[Conclusions]1. After artery injuried, PAPP-A expression increased in intimal and medial layer, and was consistent with intimal hyperplasia process.2. CD40L in vessel tissue increased after injury, it reached the highest point at 1 week, then decrease, simvastatin could inhibit vascular intima proliferation possibly by downregulated the expression of PAPP-A and CD40L.