Primary Study On Biological Activity And Mechanism Of Action Of TRAIL In HeLa Cells

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the membrane-binding cytokines that belongs to the family of tumor necrosis factor (TNF). TRAIL has been shown to be a potent apoptosis inducer in a wide variety of cancer cells in vitro and to limit tumor growth efficiently in vivo without damaging normal tissues dependent on or not p53. These functions of TRAIL suggest that it might be a potential therapeutic agent to treat human cancers.However, up to date, the role of TRAIL in cervical cancer is not very clear except functional studies of certain proteins. It's difficult to thoroughly explain the role of TRAIL, in a particular time and specific space. In this paper, first , we have cloned cDNA of human TRAIL, and obtained TRAIL protein in the prokaryotic expression system . Next, with HPV18-positive cervical cancer cell lines HeLa as model, we have studied the biological activity of TRAIL, target proteins by Comparative Proteomics. The overall results laid a solid theoretical foundation for further research on the role and mechanisms of TRAIL .Our study is composed of four contents.Part I Cloning of human soluable TRAIL and expression in prokaryotic expression system .By means of genetic engineering technology, TRAIL codons 114～118 gene were amplified by RT-PCR, transformed into coli BL21 (DE3), and...