Construction Of HIV DNA Vaccine Based On Lambda Phage-vector And A Study Of Its Preliminary Application

Human Immunodeficiency Virus(HIV) is the cause of Acquired Immunodeficiency Syndrome(AIDS). HIV is divided into two types, HIV-1 and HIV-2.HIV-1 has disseminated broadly all over the world and it is the main cause of AIDS. The HIV epidemic is still in its early stages, and a marked increase in global prevalence is projected for the next 20 years. Neither a prophylactic vaccine nor current antiretroviral drugs are likely to have an impact on the epidemic over the next decade. Current antiretroviral drugs are too expensive for the developing countries, and there are major problems of adherence,resistance and toxicity,which limit their application and efficacy. Hence, there is a great need for a therapeutic modality that does not suffer from these disadvantages. This modality would be relatively inexpensive, would be administered on a few occasions in order to mitigate problems with adherence, and would not be associated with significant toxicity or host resistance. A successful immunotherapeutic vaccine might meet these criteria.Considerable efforts have been made to develop a safe and effective HIV vaccine.Though no study has convincingly demonstrated which immune mechanism will protect against HIV infection,more and more researchers have realized that a promising HIV candidate vaccine should be capable of inducing both a strong...