Real-time PCR Detecting For Norovirus And Immune Effects Of Its Capsid Recombinant Adenovirus

Calicivirus are one of zoonosis pathogen and the most common causes of gastroenteritis in all age groups and large outbreaks remain an important public health problem worldwide. Norovirus and saporovirus were comprised to human calicivirus, due to the non- bacterium diarrhea outbreak in hospital, restaurant, school, nursery, gerocomium, armed forces, family and other crowd. Different genogroup were divided according to nucleotide and amino acid sequence of RNA polymerase and capsid, GGâ… and GGâ…¡of norovirus and SGâ… and SGâ…¡of saporovirus could infected human. Acute gastroenteritis by norovirus could autotherapy with vomit, diarrhea, abdomen, could be death if not treat promptly.The importance of calicivirus was underestimated due to the limit of detection method and it was recognized gradually with the high sensitivity diagnostic assay of RT-PCR. The studies for norovirus in our country were relatively late, and showed the infection with norovirus was at large, and it was the important pathogen causing diarrhea among children. However, the studies in our country were mainly focused on the detection of pathogen and antibody, and the results were short of representative, and were also limited. There was no clinical information and other epidemiological data, also.Hence, we want to reveal the incidence, clinical characteristic, molecule biology characteristic of calicivirus infection in Shandong. We want to establish a Taqman based real-time RT-PCR assay for the genogroupâ…¡(GGâ…¡) norovirus RNA in stool. Study the immune effect of the four genogroupâ…¡of norovirus capsid with recombinant adenovirus (rvAdGâ…¡4). It will be favor of the development of detection reagents and effective vaccine. The result of experiment as follow:1 Characterization of human calicivirus in infants with acute diarrhea in Jinan area, China. To characterize the prevalence and genetics of human calicivirus (HuCV) infection in infants with acute diarrhea in Jinan area. The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR. HuCV was detectable in 62 cases out of the 212 specimens (29.25%). And the peak of epidemic was February every year.The amplified cDNA fragments were cloned into pMD18-T vector, sequenced and...