Study On The Distribution Of Integrons And Biofilm Among Pseudomonas Aeruginosa

Pseudomonas aeruginosa is an important opportunistic pathogen of human with high drug resistance. Recent researches have revealed P. aeruginosa readily forms biofilm, which displays high resistance to antibiotics. And most of P. aeruginosa clinical isolates tested carried integrons, which played important roles in the spread of antibiotic resistance gene and multidrug resistance. The aim of this study are to detecte the distribution of integrons among P. aeruginosa clinical strains and to approach the mechanism of integron in their biofilm.In this study, the distribution and characterization of the class 1,2 and 3 integrons, among 82 P. aeruginosa clinical strains from Shijiazhuang and Kunming, were detected with specific primers of class 1 integrase gene (intI1), intI2 and intI3 by multiplex- PCR. In the result, 51 (62.2%) were positive for intI1, one was positive for intI2 and no one positive intI3 among these strains. Among 51 intI1 -positive strains, 28 (54.9%) were amplified by primers of in-F and in-B which were set for amplifying the region of resistance gene cassettes. Concerning to the antibiotic resistance, the isolates...