Construction Of Multi-gene Expressing DNA Vaccines Of Influenza A Virus And Their Immune Effects

ObjectiveInfluenza virus is the pathogen of influenza, which is a kind of highly contagious disease with the first incidence rate among all of the infectious diseases. In 20th century, 4 pandemic ever swept the global, just the 1918 Spanish influenza pandemic led to the death of at least 20 million people worldwide. Severity of influenza epidemics is a formidable public health challenge as well as significant socio-economic burden. The indulge in wilful persecution of the avian influenza from 1997 has been waking people as alarm bell. Till now people could not control the disease due to the high variability of influenza virus in spite of the rapid progress of the modern scientific technology. DNA vaccine has become the focus of widespread interest, not only because of the novelty and simplicity, but also because of its efficacy to induce broad-spectrum immunity.Haemagglutinin(HA)and neuraminidase(NA) are two major antigens on the surface of influenza virus. HA is the major protective antigen of influenza virus to stimulate the production of neutralizing antibody. Comparison with HA, NA is conserved in a degree, which stimulates the production of antibody as same. The anti-NA antibody plays the role to inhibit the virus infection. The most commonly used vaccines against the influenza virus comprise purified preparations of hemagglutinin and neuraminidase, and are designed to induce anti- hemagglutinin and anti- neuraminidase antibody. It was shown that naked DNA encoding HA or NA could induce specific...