Study On The Novel Nanoliposomal P27～(kip1) Gene Delivery System

The completion of human genome project boosts the discovery of functional genes and disease-related genes. Human being has been getting more and more understandings of us in medicine and physiology, which booms the development of gene therapy. Gene therapy has become the most active research area in biomedicine in 21^st century.With the more and more understanding of the molecular mechanism of tumors, it is recognized that the form and development process of tumors is very complex, which includes many factor, steps and genes. While the abnormity of cell cycle and the inability of cell apoptosis are two of the most critical molecular events. Cumulative research showed that the low expression of of p27^kip1 is closely related to the occurrence and bad prognosis. p27 could be act as a kind of therapeutic genes for lung gene therapy. However, the therapeutic research of p27^kip1 gene on murine lung cancer mediated by novel nanoliposomal gene delivery system has not been reported until now. In spite of the remarkable progress of gene therapy in recent 20 years, many technological problems are hindering the further success of gene therapy, and one of them is the efficient delivery of therapeutic genes. Current gene delivery systems include viral vectors and non-viral delivery systems. Viral vectors hold high transfection efficiency and poor biosafety. In contrast, non-viral delivery systems are much safer and easy to large-scale production, among which cationic liposomes/DNA complexes (LPPs) developed best. However, LPPs cannot encapsulate genes interest inside and have poor stability. In addition, the undesirable in vivo transfection results of LPPs...