Multilineage Differentiation Of Adipose-derived Stromal Cells And The Application In The Tissue Engineering

Current tissue-engineering approaches are being developed to repair and restore the function of damaged or diseased tissues. The basic principle involves an appropriate cell source and a biocompatible and biodegradable scaffold. Adipose tissue is derived from the embryonic mesenchyme and contains a stroma, in which the ASCs can be induced to multiple lineages in specific culture system similar to bone marrow stem cells (BMSCs). Compared with BMSCs, ASCs are easier to obtain, carry relatively lower donor site morbidity, and are available in large numbers of stem cells at harvest. There is less cell heterogeneity in ASCs, but apparent heterogeneity in BMSC. So it is suggested that population of ASCs represents a promising alternative for BMSCs used in cell-based musculoskeletal tissue-engineering strategies.In the part one, we aimed to confirm the trans-germ plasticity of endogenously GFP marked ASCs from GFP transgenic mice. The study was focused on two aspects. The first one was to determine whether GFP transgenic ASCs still could be induced to trans-germ multiple lineages in vitro. The second was aimed at the verification that the inductive conditions and the progress of terminal differentiation had no significant influence on GFP expression. We showed that ASCs isolated from transgenic mice have the capability to differentiate into adipogenic, osteogenic,...