Differentiation Of Adult Mesenchymal Stem Cells To Odontoblast-like Cells By Transfection Of Mouse Dentin Sialophosphoprotein Gene

The research of tooth regeneration is a front topic in current stomatology medical domain. Today, tooth regeneration is becoming possible step by step with the modern scientific advancement in cell biology, molecular biology and material science, as well as following the idea of tooth tissue engineering, Meanwhile, more and more scientists show their interest in this field. At present, the research of tooth regeneration is focused on which cell has potential capacity for odontogenesis and how the differentiation of seed cells toward odontogenesis can be induced. In addition, compatible materials for scaffolds play an important role in the construction of tissue engineering organization. As the representative of adult mesenchyma stem cells, we chose bone marrow derived mesenchymal stem cells (BM-MSCs) and adipose tissue derived stromal cells (ADSCs) for our tooth regeneration research. By gene transfection with adenovirus, BM-MSCs and ADSCs were high expressed mouse dentin sialophosphoprotein gene in vitro. We simulated the internal environment of odontablast differentiation in vivo and explored a way to induce mesenchyma stem cells orient differentiation by importing exogenous...