The Study Of NES1 Expession And Its Exons 3 CpG Methylation In Eyelid Basal Cell Carcinoma

Purpose (1)To investigate the expression of NES1 gene and protein in eyelid basal cell carcinoma (BCC),and analyze the correlation between its expression and the tumorigenic phenotype. In addition, to study the role of NES1 in oncogenesis and progression of eyelid basal cell carcinoma at gene and protein level, and estimate the feasibility as a tumor marker in the early diagnosis and prognosis.(2) In order to find out the mechanisms of loss of NES1 expression, we have studied the methylation condition in exon 3,and expect to find out a new target in the treatment of eyelid BCC.Methods The experiment has two parts. In the first part, we have studied the expression of NES1 gene and protein in 31 cases of eyelid basal cell carcinoma by using real-time quantitative PCR and immunohistochemistry. and analyzed the correlation between its expression and the tumorigenic phenotype at gene and protein level. We quantificationally analyzed the expression of hk10 in different groups by using Image-Pro Plus5.0 soft. In...