The Phenomenon And Mechanism Of Insulin Resistance In Cardiomyocytes During Calcium Overload

Our previous animal experiments showed that accompanied with peripherial insulin resistance, acute myocardial insulin resistance developed after ischemia reperfusion during cardiac operations with cardiopulmonary bypass (CPB), and the magnitude of myocardial insulin resistance was closely related to the heart dysfunction occurrence. Because the elevated anti-insulin hormones and cytokines during CPB as well as the heart functional change can affect the post-ischemic myocardial glucose uptake, so we find that ischemia-reperfusion (I/R) can induce acute myocardial insulin resistance by using I/R model with cardiomycytes. Recent research have show that an increase in cytosolic free calcium concentrations ([ca2+]i) may render adipocytes less sensitive to insulin. It is no finding that an association of elevated intracellular calcium ([ca2+]i) with insulin resistence of cardiomycytes in international documents. So, it is necessary to use the different magnitude of calcium overload model to evaluate whether calcium overload with cardiomyocytes can induce acute myocardial insulin resistance.Objective: To accurately evaluate an association of elevated intracellular calcium ([ca2+]i) with insulin resistence of cardiomycytes, as...