Functional Characterization Of Macrophages Infiltrated In Gastric Cancer And A Preparation Of Cells Transduced With Gene Of IL-2

Tumour— infiltrating macrophages (TIM) may become one of the effector cells for adoptive immunotherapy for cancer, metastatic in particular, since macrophage acts as a potent anti— tumour cell with a wide spectrum of tumour susceptibility. Functional characterization of TIM in human malignancy including gastric caucer is currently needed.Transduction with a gene of the lymphokines renders the immune cells more effective against tumour either in vivo or in vitro. The lymphokine gene therapy is thought to be a masterpiece of combining the modern science and technology with an excellent design.The aim of present study is to investigate the biological and im-munological functions, mechanism of killing in particular, of TIM in gastric cancer to see the possibility of TIM being used in adoptive immunotherapy. Another part of our study is to establish a retro-virus packaging cell line transduced with gene of IL —2 ,so that the virions carrying IL — 2 gene can be produced for transduction to immune cells.I . Isolation and purification of TIM in gastric cancer:surgical specimens from 22 patients with gastric cancer were cut, digested...