Studies On Pathology Changes And Related Cytokine Regulatory In Myocardium Compared Between Chronic Rheumatic And Traumatic Mitral Regurgitation

Objective: In order to reveal mechanism of rheumatic mitral regurgitation and trauma mitral regurgitation in protein and gene level, we studity myocardial pathology of RMR and TMR which caused chronic myocardium changes and related cytokine changes, and compared with clinical information to explor the relationship between myocardial fibrosis and heart function , and to find the criterion of myocardial fibrosis with serodiagnosis..Methods: Left ventricle papillary muscles were obtained from rheumatic heart disease during mitral valve replacement and also from canine traumatic mitral regurgitation model. The specimens were stained by HE stain, Masson stain, im-munhistochemical stain and in situ hybridization to examine myocardium pathology and related cytokine changes caused by two different nosogenesis, and to be correlating analyzed with procollagen concentration in clinical patients serum.Results: ①Myocardium interstitium hyperplasia markedly in RMR myocardium and the degree of fibrosis was as 3.28～3.72 times as normally. Vessel endothe-liosis and vascular wall thickness with lumen stenosis also appeared. The positive expression of type I and type I collagen and procollagen mRNA either appeared in interstitium cells or in myocardium cells. It suggested that cardiomy-ocyte also involved interstitium fibrosis. In the early phase of TMR collagen in myocardiac was normal or decreased and the vascular density inereased. My-...