| Background:The success of allotransplantation has paradoxically led to a shortage in the supply of organs required to meet the increasing demand. xenotransplantation could potentially overcome the organ shortage that currently limits the field of transplantation. Because of their breeding characteristics as well as their size and physiologic similarities to humans, miniature swine have been chosen as possible xenograft donors. One major barrier to organ grafting from pig to man is the presence in human serum of preformed natural antibodies (NAb) reacting with antigens expressed on porcine endothelial cells and causing hyperacute rejection, So efforts have been directed at understanding the participation of NAb in xenograft rejection. In different species combinations, the role of NAb might be different. In some cases, NAb plays a critical role, and in other cases effect of NAb is not critical. And in cells, tissues, and organ transplantation in which vascularization took place in different manner, the role of NAb might be also different. In the rat-to-rabbit model, whether or not rabbit anti-rat natural antibodies play a key role in xenograft hyperacute rejection is still unknown. Objective: To evaluate the effect of rabbit anti-rat natural antibodies on rat-to-rabbit transplantation of bone marrow cells, thymus tissues or heart. Methods : â‘ Rat bone marrow transplantation and thymus engraftment were performed following conditioning with a regimen of splenectomy + thymectomy + cyclophosphamide (50mg/kg/Dieb.alt) + goat anti-rabbit lymphocyte immunoglobulin. â‘¡ Rat-to-rabbit heterotopic transplantation were...
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