Study On The Effect Of Propofol On Ventilator-induced Lung Injury In Rats

Objective: To evaluate the effect of propofol on proinflammatory cytokines in brenche-alveolar fluid and the pathiology of lung tissues during ventilator-induced lung injury in rats. Methods: These experiments were assigned to three parts based on the three purposes for the investigation. Part one Pathology alterations during contining infusion of propofol on essential organ in rats. Twenty-one healthy male and female Sprague-Dawley rats with 250-300 gram weighing were randomly divided into 3 groups: â‘  A group (control group): without propofol injection; â‘¡ B group (venous inject propofol (18mg.kg-1.h-1) continuous for 2 hours at a fraction of inspired oxygen of 21%); â‘¢C group (venous inject propofol (18mg.kg-1.h-1) continuous for 12 hours at a fraction of inspired oxygen of 21%); â‘£D group (venous inject propofol (18mg.kg-1.h-1) continuous for 24 hours at a fraction of inspired oxygen of 21%). Some organs were taken out such as heart, pulmonary, brain, kidney, pancreas, liver and spleen after the rats were sacrificed at the end of propofol injection. And then to measure their Pathologic alterations each organ. Part two Effects of propofol intravenous anesthesia on concentration of Proin- flammatory Cytokines in Alveolar macrophages during mechanically ventilated rats. After administration of chloral hydrate, ninety healthy male and female Sprague-Dawley rats were used with the 250-300 gram weight as 6 groups (15 rats for each group) as follows: â‘ control group (group A): without mechanical ventilation or propofol; â‘¡high tidal volume group (group B): VT 34 ml/kg. â‘¢low tidal volume group (group C): VT 8 ml/kg. â‘£spontaneous (group D) for 2 h at a fraction of inspired oxygen...