The Relationship Between B Lymphocyte Stimulator And Its Receptors And Diseases

To investigate the relationship between B Lymphocyte stimulator and its receptor and diseases, we set up a real-time fluorescence quantitative Polymerase chain reaction (RFQ-PCR) method to measure the expression of BLyS and its receptors gene in peripheral blood mononuclear cells (PBMCs) based on TaqMan methodology, and detect the levels of BLyS and its receptors mRNA in patients with systemic lupus erythematosus (SLE) and multiple myeloma (MM) for the first time. In addition, we take the lead in investigating the relationship of BLyS and its receptors mRNA expression levels in patients with SLE and MM, and correlated these levels with laboratory and clinical features. We construct the 5'- deleted flanking region of human BAFF-R gene and establish a basis for the further study on the mechanism of transcription and regulation of BAFF-R gene.