The Experimental Study Of The Adipose Mesenchymal Stem Cells As The Seeding Cells Of Bone Tissue Engineering

The seeding cells are one of the keys of bone tissue engineering. It is hypothesized that human adipose tissue from liposuction is an abundant reservoir of adults stem cells, as the seeding cells of bone tissue engineering which have potentent to differentiate into osteoblasts. This research was designed to isolate, culture adipose mesenchymal stem cells (AMSCs). To observe it's capability to proliferate and the muiltipotent to differentiate to adipocytes and osteoblasts in vitro. The techniques of histiochemical stain, immunofluorescence stain and RT-PCR were used to determine the adipogenic and osteogenic differentiation. After the detection of the surface markers of the AMSCs and BMSCs by Flow cytometry, AMSCs were involved in investigation of the biological effects of basic fibroblasts growth factor(bFGF) and bone morphogenic protein on the transformation and proliferation. At last to investigate the growth and adhesion the osteoblasts-derived from adipose tissue seeded on the natural coral, the cell-scaffold complex were observed under scanning electron microscope in vitro and implanted into the node mice subcutaneously in vivo. The results had demonstrated that AMSCs were able to proliferate and differentiate into adipocytes and osteoblasts in vitro. The biological characteristic of it was similar to BMSCs' except the expression of CD73 and CD117. bFGF and BMP-2 can enhance the AMSCs differentiated into osteoblasts. From 1 day cells grew into the porous coral. TO 14-28 days, cells overgrew and many secretory collagen fiber bundles and mineralized nods were observed. The typical bone formation was not observed 8 weeks after implantation. There was few of cartilage-like and bone-like tissue but some of adipose tissue. The...