Phosphorylation Of High Mobility Group Protein Ⅰ By Protein Kinase C And Its Inflences On The Transcriptional Regulation Of Platelet-derived Growth Factor-B Chain Gene

The high mobility group protein I(HMGI), a non-histone chromatin binding protein, participates in the transcriptional regulation of numerous genes in vivo by acting as an architectural transcription factor and is involved in various biological processes including cell growth, differentiation, apoptosis and neoplastic transformation. Previous studies have shown that it is a heavily phosphorylated protein in the nucleus, being phosphorylated by various protein kinases. However, its modification by protein kinase C(PKC) phosphorylation and the functional roles of this kind of modification have not been extensively studied.It has also been shown that the induction of platelet-derived growth factor-B chain( PDGF-B) transcription by pathogenic factors is implicated in the development of atherosclerosis lesions. But the molecular mechanisms underlying the induction of the PDGF-B transcription have not been fully elucidated.The present study was focused on the modification of HMGI by protein kinase C phosphorylation and its potential roles in the regulation of PDGF-B transcription.The ³²P-incorporation and the autoradiograph showed that in the presence...