Research On The Antispermatogenesic Mechanisms And Genetic Risks Of Gossypol Combined With Steroid Hormone For Male Contraception

To study the feasibility and the morphological mechanism of combined use of steroid hormone (methyltestosterone and ethinyl estradiol) and low dose gossypol for antifertility in adult Wistar rats. We used methyltestosterone 20mg/(kg·d),ethinyl estradiol 100μg/(kg·d), and gossypol 12mg/(kg·d) to feed male rats for 6 weeks via gastric intubation to induce infertility, then used gossypol 12mg/(kg·d) alone for another 12 weeks to maintain infertility. Testicular sperm counts, cauda epididymal sperm motility and sperm silver stainning were used to determine infertility and morphology of sperm at the end of the two phases. Testicular morphology of the rats was observed from HE and PAS staining of paraffin sections and methacrylate-embedded sections. F1 generation of the rats would be examined in a series of behavioral tests after their 6-week recovery periods. There was no substantial change in testicular sperm counts of drugs-treated rats both for 6 weeks and 6 plus 12 weeks compared with that of control. But cauda epididymal sperm motility of drugs-treated rats went down to zero at the end of two phrases. The result of sperm silver...