The Protein Overexpression Of The Tumor Suppressor Gene P53 And Prognosis In Lung Cancer Patients Surgically Treated

This study was designed to determine the incidence and characteristics of the mutant of the tumor suppressor gene p53 in Chinese lung cancer patients (NSCLC & SCLC) and to explort the possible association between the mutant of the p53 gene and the c 1 inicopatholgic feature, especially the postopertive prognosis. Data thusobtained might be served as a guid to predict thelong- term outcome to install postoperativeadjuvant therapy. Monoclonal antibody to human p53protein (pAbl801 & DO-1) and immunohistochemicalmethods were used to detect p53 proteinaccumulation in formalin-fixed, paraffin-embeddedas well as fresh-frozen tissue specimens of lungcancer (202 cases of NSCLC and 32 cases of SCLC). 121 out of 202 NSCLC (59.9%)were p53 positive, including 63 out of 98 (64.3%) squamous carcinoma,41 out of 75 (54.7%) adenocarcinoma, 16 out of 26(61.5%)adenosquamous carcinoma and 1 out of 3 large cell carcinoma. Nuclear p53 protein was also observed in 81. 3% of 32 SCLC. ABC is a simple, effective, accurate and reliable method of screening the p53 protein overexpression of lung cancer. The p53 positive rate of various TNM stage in whole gruop was as follows: 51.6%...