The Construction Of Ribozymes That Specifically Cleaving The HPV16 Viral Oncogenes E6 And E7 MRNA And A Cell And Molecular Biology Research For The Reverse-transforming Effects On Human Cervical Carcinoma Cell

Human papillomavirus(HPV) is a kind of small DNA virus( φ 50-55nm) with a core comprised of 7.9Kb. The genomic DNA of HPVs are consist of early genes (E1,E2,E4,E5,E6 and E7) and late genes (L1 and L2). Up to date about 100 types of HPVs have been cloned and identified. According to the different pathogenesis,they are divided into two groups. One is the high-risk HPVs including HPV16, HPV18, HPV33 etc,which are associated with the malignant lessions, such as the carcinoma of cervix,penis,prostate, esophagus and lung etc. The other group is the low-risk HPVs including HPV6,HPV11 etc, which cause the benign lessions,such as Common Warts of skin and condyllomas of genital tract etc.The squamous cell carcinoma of the uterine cervicix is one of the most common cancers in Chinese women. In order to demonstrate the relationship between the HPVs and carcinogenesis of cervical cancer, a series of works had been done in this lab. 1150 biopies of cervix cancer, chronic cervicitis, CIN(cervical intraepithelial neoplasia), cervical condylloma and normal cervical epithelium collected from the different geograpic regions in China with different cervical cancer mortalities were investigated with the probes of HPV6,HPV11,HPV16 and HPV18 by southern blot hybridization and PCR. HPV16 are presented in 82.6% of the cervix cancer. The early genes E6-E7 are most frequently found in cancer cells DNA and have been proved to be integrated in the genomes of cancer cells. Therefore the early genes E6 and E7 are considered to be the transforming genes of HPV16. It showed there was a closed relationship between HPV16 and carcinogenes of cervical cancer in China women.