Expermental Study Of Plasmid-Mediated Intravascular Local Gene Transfer Using Protein-coated Metallic Stent Preventing Restenosis After Angioplasty

Intracoronary stent can significantly reduce the rate of restenosis following coronary angioplasty. However its efficacy is still limited by recurrence rate of 15%-20% in the target lesions, which is still the major problem unsolved in coronary interventional therapy.In order to approach a new method to prevent restenosis, our research group has completed the studies on protein coating of the metallic stent, including the biocompatibility and thrombogenicity of the protein coating of stent, the feasibility, efficiency and selectivity of adenovirus-mediated reporter gene (Lac Z gene) transfer to local arterial wall by protein-coated metallic stent as well as the effect of local prourokinase gene transfer using protein-coated metallic stents on platelet deposition, early thrombosis and smooth muscle cell proliferation after stent implantation. Becaurse the carrier of adenovirus cannot be used in the treatment of cardivouslar disesease in human now, so we carried out...