Expression Profiles Analysis Of The Toxic Effectin Babl/c Mouse LiverExposured To Four Drugs

The adverse drug reaction (ADR) is a difficult medical problem harmed the humanity health since long ago.The diseases caused by ADR in various countries are on the rise at present , furthermore, ADR is a huge barrier for the the new medicine research and clinical exertion.It has important medical and social significance for carrying on further research on toxic mechanism of medicine. Whereas,there are littler synthetical analyse measure on the molecule level in the traditional medicine toxicology, which result the slowly developing in medicine toxicology studying .Toxicogenomics supply a new technological means and research idea for the medicine toxicology. Gene chip ,which is a high flux genome-based technology , can observe simultaneously thousands gene expression model induced by drug toxic effect, and the toxicity endpoints appeared from the gene expression profile generally are more sensitive and differential that in favor of elucidating the toxicity mechanism.This research work has extensively launched and make a lot of achievements in this respect at present. The liver is a main organ of the human drug metabolism, and the liver damages induced by drug toxicity are high percentage in clinic ADR.Therefore many correlative studying are focus on drug hepatotoxicity.The objective of this experimental study is comparely studying the gene expression profile at different time phases and dosages after drug treated, which based on the balb/c mice hepatotoxic model caused by erythromycin lactobionate, tetracycline hydrochloride, cyclophosphamide, ifosfamide ,and on the mouse toxicology gene chip , accumulating data for the drug hepatotoxicity mechanism on molecule level, and validating the feasibility of the mouse toxicology gene chip applying on drug toxicity evaluation. The experimental study was composed of three parts:⑴Establishing the balb/c mice hepatotoxic model caused by erythromycin lactobionate, tetracycline hydrochloride, cyclophosphamide and ifosfamide.⑵Establishing and evaluating the technique of detection for the mouse...