Study On Damage Of Mitochondrial DNA COX Ⅰ Gene And The Effectiveness Of Rg1 On Transcriptional Factors Regulating MtDNA Expression Of Small Intestinal Epithelial Cells In Hemorrhagic Shock Rats

Hemorragic shock can introduce the damages of tissue and organs, especially, the small intestine. The small intestine served as an important target organ in hemorragic shock.Ischemia-hypoxia and the energy metabolism disorder played an important role in the pathogenesis of irreversible shock and multiple system organ failure. Mitochondria are an organelle for energy transformation. It can maintain the energy metabolism of cell, its structure and function.Mitochondrial genome codes for 13 polypeptides of oxidative phosphorylation,which is completed on the respiratory chain composed of five enzymatic complexes encoded by mtDNA and nDNA together. The mitochondria of higher mammal contains its own genome and biosynthetic institution and it functions relatively independently. nDNA plays an important role in mtDNA replication, transcription, and protein synthesis and assembly. Mitochondrial translation also relies upon nucleus-encoded ribosomal proteins, synthetases and translation factors. nDNA cooperates with mtDNA in their expressions on the transcriptional level. This cooperative expression depends on transcription and replication factors, which play an important role in controlling nucleus and mitochondrial interaction and regulating mtDNA replication and expression.Modulating of gene expression is a complicated event that processed on multiple levels.Mitochondrial genome has a close relation with nuclear genome in the way of their inheritance information expression. The genic deletion and mutation of both mtDNA and nDNA can result in incoordinate interaction of nuclear and cytoplasmy and reduction of energy metabolism efficiency. Under the pathologic condition of ischema and hypoxia after severe trauma and hemorrhagic shock,it is still unclear how the transcriptional coactivator PGC-1 and transcriptional activator mtTFA and NRFs change. Whether expression change of mtDNA was due to mitochondrial dysfunction after its damage or expression changes of transcriptional regulators encoded by nuclear genome under the...